Skip to main content
. Author manuscript; available in PMC: 2019 May 23.
Published in final edited form as: Methods Mol Biol. 2018;1840:59–71. doi: 10.1007/978-1-4939-8691-0_6

Fig. 4.

Fig. 4

Response of nesprin-TS to disruption of actin filaments. Exposure of NIH3T3 fibroblasts to 10 μM latrun-culin A results in a sustained increase in nesprin-TS FRET, indicating decreased force. The same cells were tracked before and during latrunculin treatment, which allowed for normalization to the initial FRET efficiency