Fig 8. Phylogenetic tree based on the 16S rRNA and ppk sequences to evaluate the diversity within the Leptospira genus.

In addition to the 16S rRNA sequences from the 64 genomes investigated in the present study, those from uncultured strains from the Peruvian Amazon (Clade C) [26] and from insectivorous bats from eastern China [27] were added. The branches are colored according to their belonging to the four main subclades: P1 (red), P2 (purple), S1 (green) and S2 (blue), while the strains of the “clade C” are in black. For the sake of clarity, the bootstrap values are only indicated for the nodes that correspond to the major splits. A tree constructed with the ppk gene sequences is included in the dashed box for comparison. In this case, all bootstrap values less than 100 are indicated at the different nodes.