Table 1. IC50 values (pM) of SG3552-based toxins and ADCs against T.brucei cell lines and a human Jurkat cell line.
The IC50 values of toxin SG3552, toxin plus linker SG3376, a control ADC (NIP228-SG3376) and two anti-trypanosome ADCs targeting the T. b. brucei HpHbR (Tb074-SG3376 and Tb085-SG3376) against T. b brucei wild type and T. b brucei HpHb -/- (Fig 3B) were calculated. Values in bold are best-fit IC50 values, the range is the 95% confidence intervals. It was not possible to calculate accurate IC50 values for the Jurkat cell line due to lack of saturation of the cell killing assay and so all were conservatively estimated as greater than 50 nM from the data in S2 Fig. All values are shown to 3 significant figures.
| IC50 (pM) | |||
|---|---|---|---|
| Cell line | T. b. brucei | T. b. brucei HpHbR -/- | Human Jurkat |
| SG3552 | 0.14 | 0.195 | 19.6 |
| Toxin | (0.11–0.18) | (0.14–0.27) | (10.8–35.8) |
| SG3376 | 112 | 197 | >50,000 |
| Toxin plus linker | (76.7–163) | (145–268) | |
| NIP228-SG3376 | 3750 | 3000 | >50,000 |
| Control | (2610–5380) | (2260–3960) | |
| Tb074-SG3376 | 1.33 | 3270 | >50,000 |
| (1.16–1.53) | (2400–4450) | ||
| Tb085-SG3376 | 0.297 | 1390 | >50,000 |
| (0.25–0.36) | (1030–1880) | ||