Table 1. Inflammatory mediators associated with gene signaling in endothelial cells and brain capillaries exposed to tau proteins.
Transcriptomic analysis of endothelial cells from in vitro BBB model and isolated capillaries from brainstem of transgenic rats (SHR72) and control animals. RT-PCR reactions were run in triplicate with Actb and Rplp1 used as the reference genes. Minimum fold change was set at ≥ 2, ≤ -2.
| SHR72 rat model | |||
| Gene symbol | Ref. number | Protein | Fold of regulation |
| Sele | ENSRNOG00000002723 | Selectin E | 13.9 |
| Selp | ENSRNOG00000002794 | Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) | 7.6 |
| Cxcl1 | ENSRNOG00000002802 | Chemokine (C-X-C Motif) Ligand 1 | 6.3 |
| Plau | ENSRNOG00000010516 | Plasminogen Activator, Urokinase | 2.8 |
| Nppb | ENSRNOG00000008141 | Natriuretic Peptide B | 2.4 |
| Ccl2 | ENSRNOG00000007159 | Chemokine (C-C Motif) Ligand 2 | 2.0 |
| Mmp9 | ENSRNOG00000017539 | Matrix Metallopeptidase 9 | 1.9 |
| Serpine1 | ENSRNOG00000001414 | Serpin Peptidase Inhibitor, Member 1 | 1.9 |
| Tnf | ENSRNOG00000055156 | Tumor Necrosis Factor | 1.6 |
| Ptgs2 | ENSRNOG00000002525 | Prostaglandin-Endoperoxide Synthase 2 | 1.5 |
| Kdr | ENSRNOG00000046829 | Kinase Insert Domain Receptor | -1.4 |
| Mmp1 | ENSRNOG00000032353 | Matrix Metallopeptidase 1 | -2.5 |
| in vitro BBB model | |||
| Gene symbol | Ref. number | Protein | Fold of regulation |
| Sell | ENSRNOG00000002776 | Selectin L | 361.1 |
| Mmp9 | ENSRNOG00000017539 | Matrix Metallopeptidase 9 | 156.0 |
| IL-1b | ENSRNOG00000004649 | Interleukin 1, Beta | 144.8 |
| Selp | ENSRNOG00000002794 | Selectin P (Granule Membrane Protein 140 kDa, Antigen CD62) | 137.7 |
| Cxcl1 | ENSRNOG00000002802 | Chemokine (C-X-C Motif) Ligand 1 | 107.1 |
| Ccl2 | ENSRNOG00000007159 | Chemokine (C-C Motif) Ligand 2 | 74.7 |
| Cxcl2 | ENSRNOG00000002792 | Chemokine (C-X-C Motif) Ligand 2 | 41.1 |
| Sele | ENSRNOG00000002723 | Selectin E | 23.2 |
| Vcam1 | ENSRNOG00000014333 | Vascular Cell Adhesion Molecule 1 | 17.5 |
| IL-6 | ENSRNOG00000010278 | Interleukin 6 | 15.0 |
| Ccl5 | ENSRNOG00000010906 | Chemokine (C-C Motif) Ligand 5 | 14.1 |
| Ptgis | ENSRNOG00000008245 | Prostaglandin I2 (Prostacyclin) Synthase | 12.6 |
| Pf4 | ENSRNOG00000028015 | Platelet Factor 4 | 11.9 |
| Ptgs2 | ENSRNOG00000002525 | Prostagladin- Endoperoxide Synthase 2 | 11.8 |
| Mmp1 | ENSRNOG00000032353 | Matrix Metallopeptidase 1 | 7.9 |
| Serpine1 | ENSRNOG00000001414 | Serpin Peptidase Inhibitor, Member 1 | 7.8 |
| Tnfsf10 | ENSRNOG00000013269 | Tumor Necrosis Factor Superfamily, Member 10 | 5.7 |
| Icam1 | ENSRNOG00000020679 | Intercellular Adhesion Molecule 1 | 4.4 |
| Tnf | ENSRNOG00000055156 | Tumor Necrosis Factor | 3.9 |
| Cx3cl1 | ENSRNOG00000016326 | Chemokine (C-X3-C Motif) Ligand 1 | 3.6 |
| Pdgfra | ENSRNOG00000002244 | Platelet-Derived Growth Factor Receptor, Alpha Polypeptide | 3.6 |
| F3 | ENSRNOG00000011800 | Coagulation Factor III | 3.4 |
| Xdh | ENSRNOG00000007081 | Xanthine Dehydrogenase | 3.1 |
| IL-3 | ENSRNOG00000026786 | Interleukin 3 (Colony-Stimulating Factor) | 3.0 |
| Ednra | ENSRNOG00000012721 | Endothelin Receptor Type A | 2.4 |
| Tfpi | ENSRNOG00000005039 | Tissue Factor Pathway Inhibitor | 2.4 |
| Nos3 | ENSRNOG00000009348 | Nitric Oxide Synthase 3 (Endothelial Cell) | 2.3 |
| Hif1a | ENSRNOG00000008292 | Hypoxia Inducible Factor 1, Alpha Subunit | 2.2 |
| B2m | ENSRNOG00000017123 | Beta-2-Microglobulin | 2.2 |
| Faslg | ENSRNOG00000002978 | Fas Ligand (TNF Superfamily, Member 6) | 2.1 |
| IL-7 | ENSRNOG00000011973 | Interleukin 7 | 2.0 |
| Ocln | ENSRNOG00000018297 | Occludin | -2.1 |
| Agtr1b | ENSRNOG00000010640 | Angiotensin II receptor, type 1b | -14.2 |