Fig 2. Frequency of septicemic plague in the absence of primary pneumonia increases with decreasing dosing volume.

Representative lesions from mice challenged with 2,000 CFU of wild type Y. pestis in Fig 1 that succumbed to disease on day 4. Formalin fixed tissues collected at necropsy were sectioned and stained with hematoxylin and eosin (H&E). (A) 30μL group; (B-C) 20μL group, high (B) and low (C) severity scores in the lungs; (D) 10μL group. Scale bar represents 100μm (liver) or 200μm (lungs and spleen).