Fig 5. The structures of DgoT include substrate-bound outward-facing occluded and apo-, inward-facing open conformations.

DgoT crystal structures shown in electrostatic surface potential representation. (A) A cross section through DgoT in the outward-facing occluded conformation shows galactonate (sticks) bound within the substrate recognition site (dashed white circle). The electrostatic potential of the periplasmic surface is shown below. (B) A cross section of DgoT in the inward-facing open conformation reveals an aqueous cavity with net positive surface charge. The electrostatic potential of the cytoplasmic surface is shown below. Both views highlight the positively charged binding site. DgoT, D-galactonate transporter.