Fig 8. The effect of epsA and epsS mutations on T2SS function are additive.

(A) All secreted proteins (silver stained gel) and (B) protease and lipase activities released by V. vulnificus WT, ΔepsA, ΔepsS and ΔepsAΔepsS strains. Arrows identify abundant proteins which are present in progressively lower amounts in culture supernatants. (C) Increased vesicle release by mutants defective in assembly of the EpsD secretin. Each lane contains an equal volume of membrane fragments concentrated 25-fold by ultracentrifugation of AKI or LB culture supernatants. Protein bands designated by arrows were identified by MS. Statistical analyses used a two-sided unpaired T-test (†, non-significant; *, significant [P<0.005]).