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. 2019 May 13;15(5):e1007731. doi: 10.1371/journal.ppat.1007731

Fig 10. Membrane localization of WT and S-domain mutant ExeD secretins.

Fig 10

Inner and outer membranes of WT Ah65 and exeD- mutant AhD14 expressing either WT or S-domain mutant ExeDs were fractionated by isopycnic sucrose-density-gradient ultracentrifugation. (A) The activity of the inner membrane marker NADH oxidase across a representative gradient from AhD14/pVACD (ExeD- /ExeD) is shown. (B) The SDS-PAGE protein profile across the same gradient, identifying fractions with peak outer membrane porin intensities (arrow). (C) Secretin immunoblot analyses of the membrane fractions from Ah65 or AhD14 expressing WT or S-domain mutant ExeDs.