Fig 4. Effect of ionizing radiation on the Ser15 phosphorylation of p53 produced by PTC readthrough.

The indicated cancer cells were treated with compounds for 48 h and exposed to 4 Gy ionizing radiation 24 h prior to harvesting. Following treatment, p53 and phosphorylated p53 (pp53 Ser15) levels were measured by automated capillary electrophoresis western analysis. Vinculin and FL-53 were measured in the same capillary while pp53 (Ser15) was measured in a duplicate capillary, indicated by the box. Numbers for FL-p53 and pp53 are chemiluminescence values normalized to that of vinculin and divided by 1000.