Figure 1.
TCP4-Like Genes Promote Light-Induced Cotyledon Opening.
(A) to (C) The tcp3 tcp4 tcp10 triple mutant shows delayed light-induced cotyledon opening. Four-day-old dark-grown Col and tcp3 tcp4 tcp10 seedlings were illuminated with continuous white light (at 40 µmol/m2/s). (A) Photographs of 4-d-old dark-grown seedlings (D) or after DL4 h. (B) Cotyledon opening percentages at the indicated time points after light exposure. (C) Relative cotyledon opening percentages of Col and tcp mutants at DL4 h. Data are shown as the mean ± se.
(D) to (G) Overexpression of TCP4 results in cotyledon opening in the dark. (D) The expression levels of TCP4 mRNA in 4-d-old dark-grown Col and 35S:Myc-mTCP4#4 and #10 (mTCP4 #4 and #10) seedlings were determined by RT-qPCR analysis. Data are shown as the mean ± sd of three biological replicates (see “Methods”). (E) Images of mTCP4#4 and mTCP4#10 dark-grown seedlings (4 d) showing open cotyledons. Cotyledon opening percentages (F) and cotyledon opening angles (G) of Col, mTCP4#4, and mTCP4#10 seedlings after 4 d of growth in the dark. Data are shown as the mean ± se.
Statistical analysis was performed via two-tailed Student’s t test compared with Col: ***, P < 0.001.