Figure 4.
OsGSK3 Is Involved in BR Signaling in Rice.
(A) Morphology of the osgsk3 mutant at the reproductive phase. Leaf width and leaf color of the osgsk3 mutant. Phenotypes of the osgsk3 mutant seedling. Seed morphology of the wild type and osgsk3 mutant. Bar = 1 cm.
(B) to (D) Quantification of grain length (B), tiller number (C), and 1000-grain weight (D) of the osgsk3 mutant compared with the wild type. For quantification of grain length, data are shown as means ± se (n = 30). For quantification of tiller number and 1000-grain weight, data are shown as means ± se (n = 10). The data were compared by Student’s t test. ***P < 0.001. TGW, Thousand-Grain Weight.
(E) Coleoptile elongation of the wild type and osgsk3 mutant in response to 1 μM BL. The red hyphen indicates the top of coleoptiles.
(F) Quantification of coleoptile length of the osgsk3 mutant compared with the wild type. Data are shown as means ± se (n = 20). The data were compared by Student’s t test. ***P < 0.001.
(G) Expression patterns of BR biosynthesis and signaling-related genes in the seedlings of the wild type and osgsk3 mutant. Data are shown as means ± se (n = 3).