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. 2019 May 23;8:e44649. doi: 10.7554/eLife.44649

Figure 6. Ablation of EGIns reduced neocortical spontaneous network synchronization and the frequency of mIPSCs at early postnatal stages.

(A) Schematic diagram representing ablation of EGIns and pRIns after P2. (B) Sample images showing DT administration can significantly reduce cell density of EGIns and pRIns at P5. Scale bar, 100 μm. (C) Quantitative analysis of DT ablation efficiency. (D) Sample traces showing GDPs recorded from EGIn DT-injected mice (top), pRIn DT-injected mice (middle) and wild-type DT-injected mice (bottom). Pink arrowheads indicate onsets of synchronized activities. Scale bars: 200 pA (vertical), 10 s (horizontal). (E) Quantitation of GDP frequencies in EGIn DT-injected mice, pRIn DT-injected mice and wild-type DT-injected mice. (F) Representative traces of inward mEPSCs (green traces) and outward mIPSCs (black traces) recorded in layer five pyramidal neurons in EGIn DT-injected mice, pRIn DT-injected mice and wild-type DT-injected mice. Scale bars: 20 pA (vertical), 1 s (horizontal). (G–J) Histograms of the frequencies (G) and amplitudes (H) of mEPSCs, and the frequencies (I) and amplitudes (J) of mIPSCs in EGIn DT-injected mice, pRIn DT-injected mice and wild-type DT-injected mice. Detailed statistical analysis, detailed data, and exact sample numbers are presented in the Figure 6—source data 1. Error bars indicate mean ± SEM.

Figure 6—source data 1. Detailed statistical analysis, detailed data, exact sample numbers, and p values in Figure 6 and Figure 6—figure supplement 1–3.
elife-44649-fig6-data1.xlsx (12.7KB, xlsx)
DOI: 10.7554/eLife.44649.024

Figure 6.

Figure 6—figure supplement 1. The effect of DT is specific for Cre-expressing cells.

Figure 6—figure supplement 1.

(A) Confocal microphotograph showing the cells that expressed caspase-3 in the somatosensory cortex in DT-injected CD1 mice (WT-DT, left) and saline-injected CD1 mice (WT-saline, right) at P5. (B) Comparison of the density of caspase-3 positive cells between WT-DT and WT-saline mice. Detailed statistical analysis, detailed data and exact sample numbers are presented in theFigure 6—source data 1.
Figure 6—figure supplement 2. Ablation of EGIns did not change the intrinsic electrophysiological properties of layer five pyramidal cells at P5–7.

Figure 6—figure supplement 2.

Histograms of AP threshold (A), AP amplitude (B), AP width (C) and input resistance (D) in EGIn DT-injected mice, pRIn DT-injected mice and wild-type DT-injected mice. Detailed statistical analysis, detailed data and exact sample numbers are presented in the Figure 6—source data 1. Error bars indicate mean ± SEM.
Figure 6—figure supplement 3. Ablation of EGIns did not change morphological properties of layer five pyramidal cells at P5–7.

Figure 6—figure supplement 3.

(A) Morphological samples of pyramidal cells in EGIn DT-injected mice (cell 1 and 2), pRIn DT-injected mice (cell 3 and 4) and wild-type DT-injected mice (cell 5 and 6). Scale bar, 200 μm. (B–J) Histograms of apical dendrite nodes (B), apical dendrite length (C), apical dendrite surface (D), axon nodes (E), axon length (F), axon surface (G), basal dendrite nodes (H), basal dendrite length (I) and basal dendrite surface (J). Detailed statistical analysis, detailed data and exact sample numbers are presented in the Figure 6—source data 1. Error bars indicate mean ± SEM.