Key resources table.
| Reagent type | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line | COS-7 (Cercopithecus aethiops) male |
AATC | Cat. #: CRL-1651, RRID: CVCL_0224 |
|
| Transfected construct |
KIF5C(1-560) -mNG-FRB |
(Schimert et al., 2019) PMID: 30850543 |
||
| Transfected construct |
PEX3-mRFP-FKBP | (Kapitein et al., 2010) PMID: 20923648 |
||
| Transfected construct |
GMAP-mRFP-FKBP | (Engelke et al., 2016) PMID: 27045608 |
||
| Transfected construct |
dyneinIC2-N237 | (King et al., 2003) PMID: 14565986 |
||
| Transfected construct |
KIF18A(1-452) | (Weaver et al., 2011) PMID: 21885282 |
||
| Antibody | cis-Golgi marker giantin |
BioLegend | Cat. #: 924302 RRID: AB_2565451 |
IF(1:1200) |
| Antibody | β-tubulin | Developmental Studies Hybridoma Bank |
Cat. #: E7, RRID: AB_528499 |
IF(1:2000) |
| Antibody | goat anti-rabbit Alexa680 |
Jackson Immuno Research Labs |
Cat. #: 111-625-144, RRID: AB_2338085 |
IF(1:500) |
| Antibody | goat anti-mouse Alexa350 |
Molecular Probes | Cat. #: A-11045, RRID: AB_142754 |
IF(1:500) |
| Drug | rapamycin | Calbiochem, Millipore Sigam |
Cat. #: 553210 | |
| Software | GraphPad Prism | GraphPad Software Inc |
RRID: SCR_002798 | Version 7.0 c |
| Software | MATLAB, code used for single molecule analysis |
MathWorks | RRID: SCR_001622 | R2016b, PMID: 25365993 |
| Software | Origin 2017 | OriginLab | RRID: SCR_014212 | |
| Software | RStudio, code used for quantitative dispersion analysis |
Rstudio | RRID: SCR_000432 | Version 3.4.1, manuscript in preparation |
| Software | ImageJ | NIH | RRID: SCR_003070 | |
| Software | PyMOL | PyMOL Molecular Graphics System, Schrödinger |
RRID: SCR_000304 | Version 2.2.0 |
| Software | MODELLER | PMID: 8254673 | RRID: SCR_0083595 | Version 9.18 |
| Software | AMBER 14 Package | PMID: 16200636 | RRID: SCR_014230 |