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. Author manuscript; available in PMC: 2019 Oct 8.
Published in final edited form as: ACS Biomater Sci Eng. 2018 Sep 12;4(10):3471–3477. doi: 10.1021/acsbiomaterials.8b00886

Figure 7.

Figure 7.

Apoptosis assay. The hDFs cultured in 24 well plates were exposed to no hydrogels, 10% gelatin/4% mTG hydrogels and 10% gelatin/10% mTG hydrogels through the transwell inserts over 7 days. Cell nuclei were stained blue with DAPI, apoptotic cells were stained green with TUNEL assay and actin cytoskeleton was stained red with rhodamine-labeled phalloidin. The positive control was generated by treating the no-hydrogel samples with DNase I. The composite images were generated by merging all three channels (blue, green, red) using imageJ.