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. 2019 Apr 29;8:e46976. doi: 10.7554/eLife.46976

Figure 2. Differentially translated mRNAs in Hri–/– EBs and iron deficiency.

(A) The mRNAs that are significantly differentially translated between Hri–/– and Wt EBs in +Fe or –Fe conditions. (B) Volcano plot of mRNAs that are differentially translated between Hri–/– –Fe and Wt –Fe EBs. Red dots on the positive end of the X-axis indicate significantly differentially translated mRNAs that were upregulated in Hri–/– –Fe EBs, whereas red dots on the negative end of the X-axis indicate significantly differentially translated mRNAs that were upregulated in Wt –Fe EBs. Green and gray dots indicate mRNAs that have no significant difference in translation. TE, translational efficiency. (C) Ribosome occupancies, as visualized using Integrative Genomics Viewer (IGV), of Atf4 mRNA, with an enlarged view shown in (D). (E) ATF4 protein expression in E14.5 FL cells. Ratios of ATF4/β-actin expression are indicated.

Figure 2.

Figure 2—figure supplement 1. Translational regulation of ISR mRNAs by HRI.

Figure 2—figure supplement 1.

(A) Increased translation of ISR mRNAs in Wt +Fe EBs compared to Hri–/– +Fe EBs. Red dots on the positive end of the X-axis indicate significantly differentially translated mRNAs that are upregulated in Hri–/– +Fe EBs compared to Wt +Fe EBs, whereas red dots on the negative end of the X-axis indicate mRNAs that are upregulated in Wt +Fe EBs compared to Hri–/– +Fe EBs. Green and gray dots indicate mRNAs that have no significant differences in translation. Genes labeled in blue indicate ISR mRNAs. TE, translational efficiency. (B–C) IGV-illustration of ribosome occupancies of the Ppp1r15a and Ddit3 mRNAs. Mapped reads of mRNA-seq data from Wt +Fe EBs only are shown because no significant change in mRNA levels was observed among four samples.