Figure 3. PHGDH expression in cultured Müller cells and retinal tissue from macula and periphery.

(A) Representative immunoblot showing PHGDH protein expression in primary cultures of human Müller cells from macula and peripheral retina; (B) Quantitative analysis of PHGDH relative to β-actin for immunoblots (n = 4 donor retinas); (C) Schema of trephined retina area used for Western blotting shown in (F); (D-E) Quantitative analysis of the expression of PHGDH correlated to different areas of human retina (five donors); (F) Expression of PHGDH in different retinal locations shown using western blotting (n = 5 donors).

Figure 3—figure supplement 1. PHGDH expression in the macula and peripheral retina.

(A) PHGDH expression in different retinal locations was assessed with western blot (n = 5 donors). (B) Quantitative analysis of PHGDH expression (normalized to total loaded retinal protein) was correlated with different areas of human retina (n = 5 donors).