Author response image 1. Heterogeneous expression of Syn II at GC-MLI synapses.

(A) Domain structure of mammalian Syn IIa and Syn IIb. The red and blue bar represent the domains used as immunogen to produce Millipore and Synaptic Systems (SS) Anti-Syn II antibodies. (B) Representative merged images of Syn II (Alexa-488, green) /VGluT1 (Alexa-55, red) immunostaining using Millipore anti-Syn II antibodies. Yellow puncta denote a presence of Syn II in GC boutons while red puncta denote an absence of Syn II in these synapses. Green puncta correspond to Syn II+ inhibitory synapses devoid of VGluT1. Images were captured in the molecular layer from a cerebellar section. The profile plot (blue line) confirms the presence of GC boutons devoid of SynII (red peaks not associated with a green peak). Calibration bar (left image): 7.5 μm. Anti-Syn II: Millipore #MABN1573, clone 19.4 Purified mouse monoclonal IgG2aκ antibody Immunogen: Purified recombinant rat Syn II Epitope: Domains A and B used at 1/500. Anti-Syn II: Synaptic Systems #106 211, clone 27E3 Purified mouse monoclonal IgG antibody Immunogen: Synthetic peptide corresponding to AA 440 to 458 from rat Syn II Epitope: AA 440 to 458 from rat Syn II used at 1/500