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. Author manuscript; available in PMC: 2019 Sep 18.
Published in final edited form as: Nat Microbiol. 2019 Mar 18;4(6):1049–1056. doi: 10.1038/s41564-019-0403-z

Figure 1. Tomograms reveal P22 intermediates at different stages of infection.

Figure 1.

(a, f) A phage obliquely attached to the minicell envelope after 3 min infection; (b, g) A phage attached to the minicell surface perpendicularly after 15 min infection; (c, h) A phage attached to the minicell surface showing extended density across the cell envelope after 15 min infection; (d, i) a phage with less than a complete genome in its capsid after 30 min infection; (e, j) a phage with an apparently empty capsid after 60 min infection. IM: inner (cytoplasmic) membrane; OM: outer membrane, including O antigen. Comparable tomograms have been obtained in at least three experiments using independent preparations of minicells and phage.