Table 1.
Cryo-ET data and parameters used in this study
| Genotype | Time of Incubation (min) |
Number of Tomograms | Number of extracted sub- tomograms |
Number of particles for class average with trans-envelope channel |
|---|---|---|---|---|
| Wild type | Unadsorbed | 35 | 450 | - |
| 3 | 30 | 235 | - | |
| 15 | 161 | 1,353 | - | |
| 30 | 229 | 3,432 | - | |
| 30-60 | 1,199 | 43,195 | 6,201 | |
| 30-60 | 78 (data collected on a Titan Krios with K2 detector and energy filter; used for the reconstruction shown in Fig. 4b) | 7,803 | 2,250 | |
| Gp16-defective | 30-60 | 247 | 19,115 | 2,886 |
| Gp20-defective | 30-60 | 224 | 10,802 | -- |
| Gp7-defective | 30-60 | 149 | 6,636 | -- |
| Sum | -- | 2,352 | 93,021 | 11,337 |