Time-response curves in panel (A) suggest that repeated IV injections of vehicle had minimal influence on cortical CBF, while multiple injections of PAME increased CBF under normal physiological conditions. Repeated injection of PAME, and vehicle had no effect on MABP. A single bolus injection of PAME (0.9 µg/kg), or vehicle (0.0026% ethanol) via femoral vein was applied every 10 mins for 40 mins. Cortical CBF was measured via LDF, while MABP was simultaneously measured via femoral artery throughout the 40-min-experiment. Results were expressed as mean ± SEM. *p≤0.05 indicates significantly different from vehicle, evaluated by linear mixed models: fixed effects. n indicates number of experiments.