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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: Prostaglandins Leukot Essent Fatty Acids. 2018 Nov 23;147:6–14. doi: 10.1016/j.plefa.2018.11.011

Fig. 5. Post-treatment of PAME (100 nM) provided neuroprotection against 45 mins of OGD-induced injury in the rat hippocampal slice.

Fig. 5.

OGD-induced neuronal cell death was determined by PI fluorescence. (A) Representative images of slices: Bright-field images following OGD in PAME- (I) and vehicle-treated slices (IV); PI fluorescence images 24 hours following OGD in PAME- (II) and vehicle-treated (V) slices. Effects of PAME, and vehicle on OGD-induced neuronal cell death were normalized with NMDA (500 μM)-induced maximum neuronal cell death (III and VI) and summarized in panel B. Post-treatment of PAME inhibited OGD-induced neuronal cell death in the CA1 region of the hippocampus. The data were expressed as percent cell death. *p<0.05 indicates significantly different from OGD + vehicle, evaluated by one-way ANOVA with Tukey’s post-hoc test. Scale bars = 500 µm.