4.

circ_0007766对SPCA-1和A549细胞周期的影响及统计分析。A：各组细胞固定后加入PI染色，进行流式细胞仪上机检测，用细胞周期拟和软件ModFit分析。分析时使用FL2-w和FL2-A模式；B：实验组细胞受阻于G₀/G₁期，该期细胞比例显著升高（^*P < 0.05, ^**P < 0.01）。

Effect of circ_0007766 on cell cycle of SPCA-1 and A549 cells and statistical analysis. A: Cells in each group were fixed and stained with PI. Flow cytometry was used to detect the cell cycle; B: The results were analyzed by cell cycle simulation software ModFit. FL2-w and FL2-A patterns were used. Cells in the experimental group were arrested in G₀/G₁ phase, and the proportion of cells in this phase was significantly increased (^*P < 0.05, ^**P < 0.01).