Figure 5.

Survival Probability Patterns of Flies Deficient in PGRP-SA or PGRP-LC (or Both) following Infection with WTA-less Bacteria

(A) Following infection with S. aureus, there was a statistically significant difference in the contribution of survival to infection when comparing either PGRP-SA^seml or PGRP-LC^ΔE12 single mutants with the wild-type control w¹¹¹⁸ (which survived more often) or with the homozygous PGRP-SA^seml; PGRP-LC^ΔE12 double mutant (which survived less often). However, PGRP-SA contributed more since PGRP-SA^seml was statistically closer to the double mutant.

(B) Infection with S. aureusΔTagO increased the statistical difference between PGRP-SA and PGRP-SA^seml; PGRP-LC^ΔE12. This indicated the augmented participation of PGRP-LC in survival.

(C) After infection with B. subtilis, the survival probabilities of flies followed a pattern in which both PGRPs participated and the double homozygote mutant displayed the highest susceptibility, while w¹¹¹⁸ displayed the lowest susceptibility.

(D) Nevertheless, removal of WTAs rendered both single mutants resistant to B. subtilisΔTagO as w¹¹¹⁸ and the double heterozygote, whereas only the double homozygous mutant displayed a moderate susceptibility to infection.

The results shown here are from pooled survival data (n = 87–100 for each genotype) from three independent sets of injections (there were no significant departures between repeats; p > 0.1, log rank test) and plotted estimates of survival probabilities. To extract p values, populations were compared using log rank and Wilcoxon tests.

See also the statistics in Table S3.