REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Monoclonal anti-HIV-1 Env PGT145	Produced in house; Walker et al., 2011	NA
Monoclonal anti-HIV-1 Env PG9	Produced in house; Walker et al., 2009	NA
Monoclonal anti-HIV-1 Env PG9 iGL	Produced in house; This paper	N/A
Monoclonal anti-HIV-1 Env CAP256.09	Produced in house; Doria-Rose et al., 2014	N/A
Monoclonal anti-HIV-1 Env CAP256 UCA	produced in house; Doria-Rose et al., 2014	N/A
Monoclonal anti-HIV-1 Env CH01	Produced in house; Bonsignori et al., 2011	N/A
Monoclonal anti-HIV-1 Env CH01 iGL	Produced in house; This paper	N/A
HIV mAbs	NIH AIDS Reagent Program or various research laboratories	N/A
Alkaline Phosphatase-AffiniPure Goat Anti-Human IgG, Fc Fragment Specific	Fisher Scientific	Cat# 109-055-098
Bacterial and Virus Strains
BG505_W6M_C2	NIH AIDS Reagent Program	N/A
MT145K	This paper	N/A
Q23_17	NIH AIDS Reagent Program	N/A
WITO4160_33	NIH AIDS Reagent Program	N/A
C1080_C3	NIH AIDS Reagent Program	N/A
ZM233_6	NIH AIDS Reagent Program	N/A
12 virus panel – global isolates	NIH AIDS Reagent Program	N/A
Chemicals, Peptides, and Recombinant Proteins
X-tremeGENE 9 DNA Transfection Reagent	Sigma-Aldrich	Cat# 6365809001
PEI MAX 40000	Polysciences, Inc.	Cat# 24765-1
Galanthus nivalis lectin (snow drop),agarose bound	Vector Labs	Cat#AL-1243
DEAE-Dextran	Sigma-Aldrich	Cat# D9885-10G
GAB1 SOSIP.664	This paper	N/A
MB897 SOSIP.664	This paper	N/A
EK505 SOSIP.664	This paper	N/A
MT145 SOSIP.664	This paper	N/A
MT145K SOSIP.664	This paper	N/A
Critical Commercial Assays
QuikChange II XL Site-Directed Mutagenesis Kit	Agilent Technologies	Cat# 200522
Bright-Glo Luciferase Assay System	Promega	Cat# E2610
Deposited Data
MT145K structure	This study	EMDB # EMD-20074 PDB # 6OHY
Experimental Models: Cell Lines
HEK293T	ATCC	Cat# CRL-3216
FreeStyle 293-F	Thermo Fisher Scientific	Cat# R79007
TZM-bl cells	NIH AIDS Reagent Program	Cat# 8129
Experimental Models: Organisms/Strains
CH01 UCA HC-only knock-in mouse model	This study	NA
Recombinant DNA
pSG3Δenv plasmid	NIH AIDS Reagent Program	Cat# 11051
Software and Algorithms
Prism v7.0	GraphPad	https://www.graphpad.com/scientific-software/prism/
UCSF Chimera	Pettersen et al., 2004	N/A
Other
Superdex 200 Increase 10/300 GL column	GE Healthcare	Cat# 28990944
Anti-human IgG Fc Capture (AHC) Biosensors	ForteBio	Cat#18-5060
Titan Krios	Thermo Fisher Scientific	N/A
K2 Summit	Gatan	N/A
Motioncor2	Zheng et al., 2017	N/A
GCTF	Zhang, 2016	N/A
Relion	Scheres, 2012	N/A
Rosetta	Rosetta Commons	https://www.rosettacommons.org/
Coot	Emsley and Cowtan, 2004	N/A