Positive staining of Bax was indicated by granular brown staining primarily located in the cytoplasm. (A) Light microscope images of the control, ADM-NP and ADM-NMC groups (magnification, ×100); scale bar, 100 μm. (B) Light microscope images of the control, ADM-NP and ADM-NMC groups (magnification, ×400); scale bar, 15 μm. ADM-NP group, adriamycin-loaded PLGA nanoparticle group; ADM-NMC, adriamycin-loaded PLGA nanoparticle microbubble complex group; Bax, B cell lymphoma-2-associated X protein; PLGA, polylactic-co-glycolic acid.