Fig. 6.

Sub-MIC of tobramycin enhance iron/heme uptake strategies in P. aeruginosa biofilm cultures. Relative pvdS, pvdH, hasI, phuR, and feoB mRNA levels in P. aeruginosa biofilm cultures exposed to sub-MIC of tobramycin (green bars) compared to the relative mRNA levels in the control condition (brown bars), after 24 h of growth. Quantifications have been obtained from at least three independent experiments and rpoD was used as a control housekeeping gene. Error bars represent standard error of the means (n = 3). Statistics were achieved by a two-tailed t test: ★★★, P = 0.0001 to 0.001; ★★, P = 0.001 to 0.01; ★, P = 0.01 to 0.05