. 2019 May 23;9:7745. doi: 10.1038/s41598-019-44102-9

Table 4.

Results for method validation.

No.	Standard	Linear regression curve	R²	LOD (μg/mL)	LOQ (μg/mL)	Recovery rate (%)^a	RSD (%)^b
No.	Standard	Linear regression curve	R²	LOD (μg/mL)	LOQ (μg/mL)	Recovery rate (%)^a	Intra-day	Inter-day
1	Gallic acid	Y = 18817X − 10155	0.9996	0.014	0.042	/^c	/^c	/^c
2	Caffeic acid	Y = 43686X − 19547	0.9998	0.010	0.030	/^c	/^c	/^c
3	Apigenin	Y = 29655X + 6320.6	0.9999	0.013	0.040	99.50 ± 2.69	1.16	2.33
4	Acacetin	Y = 26463X + 9248.4	0.9999	0.013	0.041	102.19 ± 3.10	0.68	1.54
5	Luteolin	Y = 24041X + 44178	0.9989	0.036	0.109	100.47 ± 2.96	1.84	2.70

^aRecovery rate (%) = [(measured amount after spiking − measured amount before spiking)/actual amount spiked] * 100.

^bRSD (%) = (SD/Mean) * 100, where RSD means relative standard deviation and SD means standard deviation.

^cNot analyzed.