Table 2.

Primary serum, clones, source, titer, and antigen retrieval

Primary serum	Clones	Source	Titer	Antigen retrieval
Bcl‐2	124	Dako	1 : 200	EDTA/Tris, pH 9.0
Cleaved Caspase‐3	Polyclonal	Cell Signaling	1 : 1000	Citrate buffer, pH 6.0
Survivin	Polyclonal	Neomarkers	1 : 350	Citrate buffer, pH 6.0
Par‐4	A‐10	Santa Cruz	1 : 700	Citrate buffer, pH 6.0
MUC1	Ma552	Novocastra	1 : 500	Citrate buffer, pH 6.0
PHLDA1	M‐20	Santa Cruz	1 : 100	Citrate buffer, pH 6.0
SPARC	Polyclonal	Chemicon	1 : 6000	Trilogy*
Fas	EPR5700	LSBio	1 : 700	Trilogy
FasL	Polyclonal	LSBio	1 : 700	Trilogy
Ki‐67	30‐9	Roche	Ready to use	Automated protocol (Ventana, Roche)

Fas, Fas cell surface death receptor; FasL, Fas ligand; Par‐4, prostate apoptosis response‐4; PHLDA1, pleckstrin homology‐like domain family A member 1; Bcl‐2, B‐cell CLL/lymphoma 2; MUC1, mucin 1; SPARC, secreted protein acidic and cysteine‐rich.

^*Trilogy, solution that allows deparaffinisation and antigen retrieval in a one‐step procedure.