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. 2019 May 17;6:65. doi: 10.3389/fcvm.2019.00065

Figure 1.

Figure 1

MiRNA-1 is down-regulated upon cardiac fibroblast activation in vivo from subcutaneous Ang II-infused hearts and in vitro upon Ang II or TGFβ stimulation. (A) Representative images of Picrosirius Red staining from free wall (cross sections) of left ventricles of control (saline) and Ang II-infused hearts. Scale bar: 500 μm. (B) Quantitation of Picrosirius Red staining in entire ventricular cross-section area. n = 5 rats each. Data are expressed as relative changes to Ctr. *P < 0.05 vs. Ctr. (C) Heart weight (HW) to body weight (BW) ratios. n = 5–6 rats each. * P < 0.05 vs. Ctr. (D) Real-time PCR analysis of ANF mRNA expression in freshly isolated cardiac myocytes from rats subjected to subcutaneous saline (Ctr) or Ang II infusion. Data are normalized to 18S and expressed relative to controls. n = 8–9 rats each, *P < 0.05 vs. Ctr. (E,F) Real-time PCR analysis of miRNA-1 expression in freshly isolated cardiac myocytes and cardiac fibroblasts from rats subjected to subcutaneous saline (Ctr) or Ang II infusion for 4 weeks. Data are normalized to U6 and expressed relative to controls. n = 7 rats each. * P < 0.05 vs. Ctr. (G) Real-time PCR analysis of miRNA-1 expression in adult rat ventricular fibroblasts (P1) that were treated with Ang II (1 μM) or TGFβ1 (10 ng/ml) for 48 h. n = 5–6 each. Data are normalized to U6 and expressed relative to respective controls. *P < 0.05 vs. respective Ctr.