Figure 5.

Co‐immunoprecipitation (Co‐IP) of wild‐type and mutant ANK1 with SPTB/SLC4A1 in human embryonic kidney 293T (HEK293T) cells. Co‐IP analysis of physiologically relevant ANK1‐SLC4A1/SPTB interactions in HEK293T cells using FLAG antibodies. None or little SCLC4A1/SPTB was captured when ANK1 was mutated in the three sites. The asterisks indicate non‐specific or protein degradation product. Semi‐quantitative analysis of the captured amount of SPTB/SLC4A1 relative to the amount of immunoprecipitated ANK1 was performed by greyscale scanning of strips. Data are the mean values of duplicate samples