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. 2019 Mar 28;23(6):4139–4152. doi: 10.1111/jcmm.14301

Figure 7.

Figure 7

Adenosine monophosphate‐activated protein kinase (AMPK) signalling is involved in TGF‐β1 mediation of mitochondrial dynamics in vascular progenitor cells. A, Western blotting and quantitative analysis of the level of p‐AMPK protein in control‐vascular progenitor cells (VPCs) and Marfan syndrome (MFS)‐VPCs. B, Western blotting and quantitative analysis of the level of p‐AMPK, p‐Drp1 and Mfn2 protein in control‐VPCs that received TGF‐β1 or TGF‐β1 + AICAR treatment. C, Representative images of SA‐β‐gal staining in control‐VPCs that received TGF‐β1 or TGF‐β1 + AICAR treatment. D, The SA‐β‐gal positive cells in control‐VPCs that received TGF‐β1 or TGF‐β1 + AICAR treatment were calculated and are presented as a percentage of the total cells. E, Representative images of Mito‐Sox staining in control‐VPCs that received TGF‐β1 or TGF‐β1 + AICAR treatment. F, Quantitative analysis of ROS generation in control‐VPCs that received TGF‐β1 or TGF‐β1 + AICAR treatment. Data are expressed as mean ± SEM (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001. Scale bar = 100 μm