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. 2019 Apr 13;23(6):4256–4268. doi: 10.1111/jcmm.14316

Table 1.

Primers of genes analysed by real‐time PCR

Gene symbol Forward sequence Reverse sequence Amplicon length (nt)
GAPDH 5′‐CTCTCTGCTCCTCCTGTTCG‐3′ 5′‐ACGACCAAATCCGTTGACTC‐3′ 114
CD90 5′‐CTAGTGGACCAGAGCCTTCG‐3′ 5′‐GCCCTCACACTTGACCAGTT‐3′ 96
CD105 5′‐CCTCTACCTCAGCCCACACT‐3′ 5′‐CTGTCTAACTGGAGCAGGAACTC‐3′ 92
CD146 5′‐AGCCAAACATCCAGGTCAAC‐3′ 5′‐TACCCGTTCCTCCCTACACA‐3′ 88
ECM2 5′‐ATCCTTTTCAAGTTTTCCTGGAG‐3′ 5′‐TGCCCTTTACCAAACAGTGTC‐3′ 80
FN1 5′‐ACCGAGGTGACTGAGACCAC‐3′ 5′‐GACACAACGATGCTTCCTGA‐3′ 137
WISP1 5′‐TGGCAGCAGTGACAGCA‐3′ 5′‐GGAGCTGGGGTAAAGTCCAT‐3′ 88