Figure 4.

Effect of miR‐193a/b‐3p on apoptosis and cell cycle of human hepatic stellate cells LX‐2. (A,B) Apoptosis of LX‐2 cells were detected by Terminal‐deoxynucleotidyl transferase‐mediated nick end labelling (TUNEL) (200×) and the percentage of TUNEL‐positive cells was shown. Scale bar is 100 μm. (C,D) The mRNA expression of CAPRIN1 in LX‐2 cells was evaluated by RT‐PCR. The protein levels of CAPRIN1 (E,F), Cyclin D1 (G), Cyclin E1 (H) and p‐RbS807 (I) in LX‐2 cells were assessed by western blot analysis. J, The wild‐type (WT) and mutant (MUT) binding sites of the CAPRIN1 3′‐UTR to miR‐193a/b‐3p were cloned into the pmirGLO vector. The direct binding of miR‐193a‐3p (K) or miR‐193b‐3p (L) with CAPRIN1 was determined by dual‐luciferase report system. All data were expressed as mean ± SD (n = 3). *P < 0.05, ***P < 0.001 vs the indicated group