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. 2019 Apr 14;23(6):4229–4243. doi: 10.1111/jcmm.14313

Figure 5.

Figure 5

A, Western blot and semiquantitative analyses for smad7 and p‐smad7 expression in human CFs treated with different concentrations of rTGFβ3. GAPDH and total smad were used as an endogenous control. The data are presented as the mean ± SEM. **P < 0.01 versus the 0 ng/mL rTGFβ3 group; *P < 0.005 versus the 0 ng/mL rTGFβ3 group; B, Western blot and semiquantitative analyses for smad7 and p‐smad7 expression in human CFs treated with endogenous TGFβ3. GAPDH and total smad were used as an endogenous control. The data are presented as the mean ± SEM. *P < 0.05 versus the si‐TGFβ3‐NC group; **P < 0.051 versus the si‐TGFβ3‐NC group; ##P < 0.01 versus the pEX4‐TGFβ3‐NC group; ###P < 0.005 versus the pEX4‐TGFβ3‐NC group; C, The transfection efficiency of smad7 siRNA was confirmed by RT‐qPCR. The results are shown using GAPDH as an endogenous control. ***P < 0.005 versus the si‐smad7‐NC group; D, The transfection efficiency of smad7 siRNA was confirmed by Western blot and semiquantitative analyses. The results are shown using GAPDH as an endogenous control. The data are presented as the mean ± SEM. ***P < 0.005 versus the si‐smad7‐NC group; E, F, Transwell assay and cell count analysis were used on 24‐h‐Ang Ⅱ (100 nmol/L)‐TGFβ3 (10 ng/mL) treatment and 48‐h‐si‐smad7 transfection cells to evaluate the migration effect of smad7 on human CFs. ***P < 0.005 versus the Ang Ⅱ group; ###P < 0.005 versus the rTGFβ3 group; &&& P < 0.005 versus the Ang Ⅱ+rTGFβ3 group. G, CCK‐8 assay was used on 24‐h‐Ang Ⅱ (100 nmol/L)‐TGFβ3 (10 ng/mL) treatment and 48‐h‐si‐smad7 transfection cells to evaluate the proliferation effect of smad7 on human CFs. ***P < 0.005 versus the Ang Ⅱ group; ###P < 0.005 versus the rTGFβ3 group; &&& P < 0.005 versus the Ang Ⅱ+rTGFβ3 group. H, MTT assay was used on 24‐h‐Ang Ⅱ (100 nmol/L)‐TGFβ3 (10 ng/mL) treatment and 48‐h‐si‐smad7 transfection cells to evaluate the cytotoxicity effect of smad7 on human CFs. *P < 0.05 versus the Ang Ⅱ group; ###P < 0.005 versus the rTGFβ3 group; &&& P < 0.005 versus the Ang Ⅱ+rTGFβ3 group