Figure 1.

Differentially expressed lncRNAs and mRNAs in MTX‐resistant/sensitive CRC cells (A) The top 20 up‐ and down‐regulated lncRNAs were filtrated using microarray analysis. LncRNA KCNQ1OT1 was overexpressed in MTX‐resistant CRC cells compared with its expression in MTX‐sensitive cells, as shown in the heatmap. (B) The top 20 up‐ and down‐regulated mRNAs were selected through microarray analysis. PPP1R1B was up‐regulated in MTX‐resistant CRC cells compared with that in MTX‐sensitive cells, as shown in the heatmap. (C) Coexpression network of differentially expressed lncRNAs and mRNAs. KCNQ1OT1 was found to be correlated with PPP1R1B. (D) The binding sites of lncRNA KCNQ1OT1, PPP1R1B, and the specific miRNA (miR‐760) were determined with TargetScan and miRanda