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. 2019 Apr 17;23(6):3808–3823. doi: 10.1111/jcmm.14071

Figure 5.

Figure 5

Silencing of KCNQ1OT1 influenced the proliferation of MTX‐resistant CRC cells through the sponging of miR‐760. (A) The targeted relationship between KCNQ1OT1 and miR‐760 was determined with the dual‐luciferase reporter gene assay in HT29/MTX cells under the treatment with MTX. **P < 0.01 compared with the KCNQ1OT1 +  NC group. (B) The targeted relationship between KCNQ1OT1 and miR‐760 was determined with the dual‐luciferase reporter gene assay in Caco2/MTX cells under the treatment with MTX. **P < 0.01 compared with the KCNQ1OT1 +  NC group. (C) MiR‐760 was visibly down‐regulated in HT29/MTX cells compared with its levels in HT29 cells, as determined by qRT‐PCR. **P < 0.01 compared with the HT29 cells. (D) MiR‐760 was markedly down‐regulated in Caco2/MTX cells compared with its expression in Caco2 cells, as detected by qRT‐PCR. **P < 0.01 compared with the Caco2 cells. (E) qRT‐PCR was performed to measure miR‐760 expression after transfection in HT29/MTX cells. **P < 0.01 compared with the NC group, ##P < 0.01 compared with the miR‐760 inhibitor group. (F) qRT‐PCR was performed to measure miR‐760 expression after transfection in Caco2/MTX cells. *P < 0.05 and **P < 0.01 compared with the NC group, ##P < 0.01 compared with the miR‐760 inhibitor group. (G) Cell proliferation was measured with a CCK‐8 assay to explore the effect of the KCNQ1OT1/miR‐760 axis in HT29/MTX cells. *P < 0.05 and **P < 0.01 compared with the NC group, #P < 0.05 compared with the miR‐760 inhibitor group. (H) Cell proliferation was measured with a CCK‐8 assay to explore the effect of the KCNQ1OT1/miR‐760 axis in Caco2/MTX cells. *P < 0.05 compared with the NC group, #P < 0.05 compared with the miR‐760 inhibitor group