Figure 7.

MiR‐760 modulated PPP1R1B expression and the downstream proteins CREB and CBP in the cAMP signalling pathway. (A) The targeted relationship between miR‐760 and PPP1R1B was determined by dual‐luciferase reporter assay in HT29/MTX cells under the treatment with MTX. **P < 0.01 compared with the PPP1R1B + NC group. (B) The targeted relationship between miR‐760 and PPP1R1B was determined with the dual‐luciferase reporter assay in Caco2/MTX cells under the treatment with MTX. **P < 0.01 compared with the PPP1R1B + NC group. (C) PPP1R1B was overexpressed in HT29/MTX cells, as measured by qRT‐PCR. **P < 0.01 compared with the HT29 cells. (D) PPP1R1B was overexpressed in Caco2/MTX cells, as measured by qRT‐PCR. **P < 0.01 compared with the HT29 cells. (E) Western blot was used to examine the influence of miR‐760 on PPP1R1B and the related proteins CREB and CBP in the cAMP signalling pathway in HT29/MTX cells. (F) Western blot was used to examine the influence of miR‐760 on PPP1R1B and the related proteins CREB and CBP in the cAMP signalling pathway in Caco2/MTX cells. (G) The expression levels of PPP1R1B,CREB, and CBP were measured by qRT‐PCR to explore the effect of the PPP1R1B/miR‐760 axis in HT29/MTX cells. **P < 0.01 compared with the NC group, #P < 0.05 and ##P < 0.01 compared with the PPP1R1B group. (H) The expression levels of PPP1R1B,CREB and CBP were measured by qRT‐PCR to explore the effect of the PPP1R1B/miR‐760 axis in Caco2/MTX cells. *P < 0.05 and **P < 0.01 compared with the NC group, #P < 0.05 compared with the PPP1R1B group. (I) Cell viability was determined with a CCK‐8 assay to explore the effect of the PPP1R1B/miR‐760 axis in HT29/MTX cells. *P < 0.05 and **P < 0.01 compared with the NC group, #P < 0.05 compared with the PPP1R1B group. (H) Cell viability was determined with a CCK‐8 assay to explore the effect of the PPP1R1B/miR‐760 axis in Caco2/MTX cells. *P < 0.05 and **P < 0.01 compared with the NC group, #P < 0.05 compared with the PPP1R1B group