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. 2019 Apr 23;23(6):4097–4110. doi: 10.1111/jcmm.14297

Figure 5.

Figure 5

miR‐449c is expressed in pituitary corticotroph tumours and targets the 3′UTR of TSP‐1. A, The expression of miR‐449c mRNA in Cushing's disease (n = 33) and normal pituitary tissue (n = 7) were determined by qRT‐PCR. **P < 0.01. B, The expression of miR‐449c mRNA in AtT20 cells and normal mouse pituitary, tested by qRT‐PCR. C, The predicted target sequence of miR‐449c in the 3′UTR of TSP‐1 and mutant containing three altered nucleotides in the seed sequence of miR‐449c. D, Luciferase assay of pGL3‐TSP‐1WT or pGL3‐TSP‐1 mutant in the presence of miR‐449c mimic in the AtT20 cell line. Luciferase activity was detected 48 h after transfection and normalized to Renilla. (E) qRT‐PCR and (F) Western blotting analysis of miR‐449c effects on mRNA level of TSP‐1 in AtT20 cells. **P < 0.01