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TSP‐1 reversed miR‐449c expression to affect the function of AtT20 cells in vitro. AtT20 cells were transfected with mimics control or miR‐449c and miR‐449c+TSP1. A, Invasion of AtT20 cells in different groups by wound healing assay. B, Transwell invasion assay, (C) MTT assay. (D) qRT‐PCR analysis of miR‐449c and TSP‐1 effects on POMC expression, and (E) miR‐449c and TSP‐1 effects on ACTH secretion. **P < 0.01
