Figure 4.
CD9 Fab impedes the uptake of extracellular vesicles (EVs) and nuclear transfer of their cargo proteins in various malignant melanoma cells. (A‐C) Melanoma A375 or C8161 cells were incubated (30 min) without (control) or with CD9 Fab or CD9 Ab (25 μg/mL) prior to the exposure to fluorescent EVs (2.5 × 108 particle per mL) derived from FEMX‐I cells expressing CD9‐GFP for 5 h. Samples were then fixed and immunolabelled for SUN2 prior to confocal laser scanning microscopy. Cytoplasmic (A) and nuclear (B, C) CD9‐GFP signals per cell were quantified using Fiji. Means with the range of fluorescence per slice from 10 individual and representative cells are shown (A). 30 cells were evaluated per condition and experiment (B) and the means ± SD of three independent experiments are shown (C). P‐values are indicated