Table 1.
Differential impact of CD9 antibody on the nuclear localization of extracellular vesicles (EV)‐derived cargo protein
| Antibody | Experimental procedurea | Average nuclear EV‐derived CD9‐GFP per cellc | P‐values (relative to control) | P‐values (relative to procedure A) |
|---|---|---|---|---|
| Controlb | A | 8.42 ± 0.74 | — | |
| B | 8.41 ± 0.61 | — | ||
| C | 8.02 ± 0.44 | — | ||
| CD9 Fab | A | 2.89 ± 0.13 | 0.05 | |
| B | 2.99 ± 0.45 | 0.001 | ||
| C | 1.53 ± 0.09 | 0.0001 | 0.05 | |
| CD9 Ab | A | 15.67 ± 1.20 | 0.005 | |
| B | 14.17 ± 0.76 | 0.001 | ||
| C | 14.20 ± 0.32 | 0.0001 | 0.5 |
a
A, Cells were pre‐incubated with antibody (25 μg/mL, 30 min, 37°C) before the addition of CD9‐GFP+EVs (5 h). B, CD9‐GFP+EVs were pre‐incubated with antibody (25 μg/mL, 30 min, 4°C) before their incubation with cells (5 h). C, Cells and CD9‐GFP+EVs were pre‐incubated with antibody (12.5 μg/mL each, 30 min, 37 or 4°C respectively) before their co‐culture (5 h).
b
Control refers to the three experimental procedures (A‐C) without the addition of antibody.
c
At least 30 cells were evaluated per condition (n = 3).