Figure 1.

Depletion of MICAL‐L2 inhibits EGFR expression in gastric cancer cells. A, BGC‐823 cells were transfected with control siRNA or siRNA specifically targeting MICAL‐L2 (siMICAL‐L2). Forty‐eight hours later, total protein extracts from cells were analysed for MICAL‐L2 and EGFR expression. Western blot bands corresponding to EGFR and MICAL‐L2 were quantified and normalized against GAPDH. **P < 0.01, ***P < 0.001 in the siMICAL‐L2 cells relative to siRNA control cells. B, SGC‐7901 cells transfected with control siRNA or siMICAL‐L2 were lysed, EGFR and MICAL‐L2 levels were determined by Western blotting assays. **P < 0.01, ***P < 0.001 in the siMICAL‐L2 cells relative to siRNA control cells. C, Representative immunofluorescence images of SGC‐7901 cells transfected with control siRNA or siMICAL‐L2 staining for EGFR. Scale bar, 5 μm