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. 2019 Apr 29;23(6):4475–4488. doi: 10.1111/jcmm.14353

Figure 2.

Figure 2

MICAL‐L2 regulates migration of human gastric cancer cells. A, SGC‐7901 cells were transfected with empty vector or MICAL‐L2 plasmids and the total cellular proteins were extracted and analysed for expressions of EGFR by Western blotting assays. Western blot bands corresponding to EGFR was quantified and normalized against GAPDH. **P < 0.01 in the MICAL‐L2 overexpression cells relative to control cells. B, A representative of wound healing assays in SGC‐7901 cells transfected with empty vector or MICAL‐L2 plasmids is presented and the quantification of cell migration rate was performed (n = 8 for each group). **P < 0.01 in the MICAL‐L2 overexpression cells relative to control cells. C, A representative of wound healing assays in BGC‐823 cells transfected with control siRNA or siMICAL‐L2 is presented and the quantification of cell migration rate was performed (n = 8 for each group) *P < 0.05. D, The migration capacity of BGC‐823 cells which transfected with siMICAL‐L2 was also evaluated by transwell assays. **P < 0.01 in the siMICAL‐L2 cells relative to siRNA control cells. E, BGC‐823 cells were transfected with control siRNA or siMICAL‐L2, total protein extracts from cells were analysed by Western blotting and bands corresponding to E‐cadherin, N‐cadherin and vimentin were examined. F, Cell viability of BGC‐823 cells transfected with control siRNA or siMICAL‐L2 was detected by MTT assays