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. 2019 Apr 1;23(6):3888–3896. doi: 10.1111/jcmm.14247

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© 2019 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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mRNA expression of essential genes for arteriogenesis and angiogenesis. RNA was isolated of gastrocnemius muscles of Irf3^−/−, Irf7^−/− and C57BL/6 mice sacrificed 28 days after HLI, and used for RT‐qPCR analysis. Relative mRNA expression is shown of (A) tnfα, (B) ccl2, (C) il6, (D) il1β, (E) sdf1, (F) vegf164, (G) vegfr1, and (H) vegr2. Relative expression of mRNA is shown to GAPDH of the left ischemic gastrocnemius muscle. Data is presented as mean SEM; *P < 0.05; **P < 0.01, a 1‐way ANOVA and a Kruskal‐Wallis test was used. n = 5‐8.