Figure 2.

Concomitant overexpression of triple antioxidant enzymes effectively preserved the muscle fibres of the ischemic limb. The muscle fibres reduced in size and became largely disconnected in the WT control mice with limb ischaemia. CD31 staining showed that capillary density was low in the limb muscle after ischaemia for 3, 7 and 21 days. On the other hand, the muscle fibres appeared to remain long and large, as well as well‐connected in the limb muscles at day 21 after ischaemia in the TG mice with concomitant global overexpression of SOD1, SOD3 and Gpx‐1. CD31 staining demonstrated that the capillary density remained largely intact (similar to the non‐ischemic limb). Normal: normal right limb; ischaemia; ischemic left limb. Red arrow: capillary vessels. BL: before ligation. Scale bar: 50µm