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. Author manuscript; available in PMC: 2019 May 24.
Published in final edited form as: Chem Commun (Camb). 2017 Oct 5;53(80):11072–11075. doi: 10.1039/c7cc05492k

Fig. 3.

Fig. 3

αS TMAO FRET Experiments. Left: Incubation of αS with TMAO causes protein compaction, which can be monitored by FRET. Top Right: Fluorescence emission spectra (325 nm excitation) of 1 μM concentrations of αS-CMcm62δ94 and the corresponding singly-labelled αS-CMcm62δ94 and αS-CMcm62δ94 constructs in 0 M TMAO. Deconvolution of the doubly labelled spectrum by fitting to a weighted sum of the singly-labelled spectrum are shown. Bottom Right: EFRET and corresponding inter-fluorophore distances for αS constructs under varying conditions determined using spectral deconvolution. See ESI for details.