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. 2019 May 17;10:429. doi: 10.3389/fgene.2019.00429

FIGURE 3.

FIGURE 3

The effect of the R123E mutant of DprA on binding to single-strand DNA (ssDNA). All ssDNA was labeled by biotin. The first lane of each picture contains free ssDNA without proteins. Different concentrations of DprA (1–20 μM) were combined with 0.5 μl of DNA substrate (biotin-labeled ssDNA) and incubated for 30 min at 30°C before the mixtures were loaded onto the gel. (A) Interaction between DprA and ssDNA from the R. anatipestifer coding sequence (RA-ssDNA1). (B) Interaction between DprA and ssDNA from the R. anatipestifer non-coding sequence (RA-ssDNA2). (C) Interaction between DprA and ssDNA from E. coli XL1-blue (E. coli-ssDNA). (D) Interaction between DprAR123E and ssDNA from the R. anatipestifer coding sequence (RA-ssDNA1). (E) Interaction between DprAR123E and ssDNA from the R. anatipestifer non-coding sequence (RA-ssDNA2). (F) Interaction between DprAR123E and ssDNA from E. coli XL1-blue (E. coli-ssDNA). Samples were electrophoresed on an 8% non-denaturing PAGE gel and detected by fluorography.