Figure 4.

Treatment with insulin stimulates theAkt1/mTOR/p70S6K and Akt1/P53 pathways, and LY294002 treatment decreased the stimulation of insulin at the protein level of factors involved in these signalling pathways. A, Western blot analysis results showing the protein expression of members of the Akt1 pathway after insulin treatment. B, Western blot analysis results showing the protein expression after treatment with LY294002. C, Akt1 inhibition attenuated the pro‐proliferation effect of insulin. D, Signalling pathway of insulin promoted hiHep proliferation. All data are presented as means ± SD; n = 3 samples per experimental group. *P < 0.05