Figure 4.

Rutaecarpine (Rut) inhibited the Nox4‐ROS‐ADAM17 pathway in cardiomyocytes treated by angiotensin (Ang II). Primary cardiomyocytes were treated with rutaecarpine (10 µmol/L) for 60 min prior to stimulation with Ang II for 24 h. A and B, The contents of H₂O₂ (A) and O²⁻ level indicated by DHE fluorescence intensity (B). C, Nox4 protein level. D, Nox4 mRNA level. E, ADAM17 protein level. F, ADAM17 mRNA level. G, TNF‐α protein level in the conditioned media. H, TNF‐α mRNA level. Nox4 represents NADPH oxidase 4; ROS represents reactive oxygen species; ADAM17 represents a disintegrin and metalloproteinase‐17; TNF‐α represents tumour necrosis factor α; ^# P < 0.05 vs Control group; *P < 0.05 vs Ang II group. The statistical analyses were performed using one‐way ANOVA followed by Bonferroni's post hoc test. n = 3 independent experiments