Table 5.
Bacterial adherence to immobilized fibrinogen.
| Clinical | Premature stop | Binding to solid- | Agglutination | ||||
|---|---|---|---|---|---|---|---|
| isolate | fbl-type | codon in fbl gene | MLST CC | phase fibrinogen | test | Clinical source | City |
| SL_T29 | 43g | – | 1 | +++∗∗∗∗ | Positive | Material device infection | Rouen |
| SL_R30 | 45l | – | ND | +++∗∗ | Positive | SSTI | Rouen |
| SL_V03 | 47b | – | 3 | +++∗∗ | Positive | SSTI | Strasbourg |
| SL_V74 | 45b | – | 2 | +++∗ | Positive | Bacteremia | Strasbourg |
| SL_T117 | 45f | – | 1 | +++∗ | Positive | Carriage | Kronoberg |
| SL_V51 | 45h | – | 1 | +++∗ | Positive | BJI | Strasbourg |
| SL_T118 | 40b | – | 6 | + | Negative | Carriage | Kronoberg |
| SL_T122 | 43g | – | 1 | + | Positive | Carriage | Tours |
| SL_V06 | 45c | – | 2 | + | Negative | Deep infection | Strasbourg |
| SL_R34 | 45f | – | ND | + | Positive | SSTI | Rouen |
| SL_V05 | 45g | – | 1 | + | Positive | Deep infection | Strasbourg |
| SL_V35 | 45i | – | 1 | + | Positive | Catheter related bacteremia | Strasbourg |
| SL_R44 | 47b | – | ND | + | Negative | Carriage | Rouen |
| SL_V34 | 52a | – | 4 | + | Positive | SSTI | Strasbourg |
| SL_V07 | 21a | L22STOP | 1 | + | Negative | Deep infection | Strasbourg |
| SL_V24 | 44a | L22STOP | 1 | + | Negative | BJI on material | Strasbourg |
| SL_C47 | 13a | – | 3 | – | Negative | Carriage | Strasbourg |
| SL_R59 | 13a | – | ND | – | Negative | BJI | Rouen |
| SL_R87 | 18a | – | ND | – | Negative | Carriage | Rouen |
| SL_V50 | 24a | – | 3 | – | Negative | BJI on material | Strasbourg |
| SL_R74 | 37a | – | ND | – | Negative | Carriage | Rouen |
| SL_DSM | 41b | – | 6 | – | Positive | SSTI | Unknown |
| SL_T55 | 41b | – | 6 | – | Negative | SSTI | Nantes |
| SL_V70 | 41c | – | 7 | – | Negative | SSTI | Strasbourg |
| SL_C35 | 42a | – | 3 | – | Negative | Carriage | Strasbourg |
| SL_C72 | 42d | – | 3 | – | Negative | Carriage | Strasbourg |
| SL_R72 | 42f | – | ND | – | Negative | Deep infection | Rouen |
| SL_V78 | 43a | – | 3 | – | Negative | SSTI | Strasbourg |
| SL_R41 | 45b | – | ND | – | Negative | SSTI | Rouen |
| SL_V77 | 45d | – | 7 | – | Positive | Catheter related bacteremia | Strasbourg |
| SL_R29 | 45d | – | ND | – | Positive | Carriage | Rouen |
| SL_C42 | 45e | – | 7 | – | Positive | Carriage | Strasbourg |
| SL_V31 | 47a | – | 3 | – | Negative | Urinary infection | Strasbourg |
| SL_V10 | 47c | – | 3 | – | Negative | Material device infection | Strasbourg |
| SL_V21 | 47d | – | 3 | – | Negative | Catheter related bacteremia | Strasbourg |
| SL_V48 | 52a | – | 4 | – | Negative | Deep infection | Strasbourg |
| SL_R99 | 9a | – | ND | – | Negative | Carriage | Rouen |
| SL_T10 | 9a | – | Singleton | – | Negative | BJI | Rouen |
| SL_V81 | 30a | L22STOP | 1 | – | Negative | SSTI | Strasbourg |
| SL_V08 | 40c | L22STOP | 1 | – | Negative | SSTI | Strasbourg |
| SL_C60 | 43c | L22STOP | 1 | – | Negative | Carriage | Strasbourg |
| SL_V13 | 43c | L22STOP | 1 | – | Negative | BJI on material | Strasbourg |
| SL_V27 | 43c | L22STOP | 1 | – | Negative | BJI on material | Strasbourg |
| SL_V36 | 43c | L22STOP | 1 | – | Negative | BJI | Strasbourg |
| SL_V66 | 43c | L22STOP | 1 | – | Negative | BJI on material | Strasbourg |
| SL_T13 | 45f | L22STOP | 1 | – | Negative | Endocarditis | Rouen |
| SL_V09 | 45f | L22STOP | 1 | – | Negative | SSTI | Strasbourg |
| SL_V32 | 45f | L22STOP | 1 | – | Negative | SSTI | Strasbourg |
| SL_V64 | 45f | L22STOP | 1 | – | Negative | SSTI | Strasbourg |
| SL_V79 | 45f | L22STOP | 1 | – | Negative | Deep infection | Strasbourg |
| SL_R15 | 45k | L22STOP | ND | – | Negative | Carriage | Rouen |
| SL_V38 | 46a | L22STOP | 1 | – | Negative | BJI on material | Strasbourg |
| SL_C62 | 46b | L22STOP | 1 | – | Negative | Carriage | Strasbourg |
| SL_V62 | 46c | L22STOP | 1 | – | Negative | SSTI | Strasbourg |
| SL_V33 | 45a | L22STOP | 1 | – | Negative | Deep infection | Strasbourg |
The fibrinogen (Fg) binding analysis was performed in two independent experiments measured in at least five wells. Statistical differences compared to S. aureus DU5925 (negative control) used Dunnett’s multiple comparisons test (P < 0.05). “+++”: S. lugdunensis strains that bound significantly to solid-phase Fg (ordinary one-way ANOVA: ∗∗∗∗P < 0.0001, ∗∗P < 0.001, and ∗P < 0.05), “-”: strains with binding inferior to the negative control and “+”: others strains. CC, clonal complex; ND, not determined; SSTI, skin and soft tissue infection; BJI, bone and joint infection.